Characterising the Influence of First-Year Wheat Cultivar on Pseudomonas Selection and Function in a Take-All Infected Field

Wheat, the world’s most widely cultivated crop, is highly susceptible to Take-all disease caused by soil ascomycete fungus Gaeumannomyces tritici (Gt). Crop rotations using different wheat cultivars can influence build up (TAB) in following year’s crop. The exact mechanism for this unknown, but there may be a bacterial biocontrol component associated with TAB control. We cultured and characterised Pseudomonas isolates from rhizosphere endosphere of second-year field-grown wheat, plots which, previous season, had been used culture High-TAB cultivar Hereward or Low-TAB Cadenza. Analysis two loci, wsm fecB, showed that these genes were significantly differentially first-year wheat-planting regime: was more abundant within Hereward-derived isolates, while fecB prevalent Cadenza-derived isolates. These findings supported gyrB phylogenetic analysis an vitro Gt antagonism assay. data show grown first year plays especially important role selection spp. found second-season wheat-root environment, emphasising importance understanding agronomic practice improving plant health food security.